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CD4, Mouse (T-Cell Marker) Antibody [GK1.5]

In Stock
Catalog Number Formulation Size Price
RTM1-2009-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
RTM1-2009-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
RTM1-2009-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-2ug/ml

Summary

CD4 is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase, lck. CD4 is expressed by the majority of thymocytes, most helper T cells, a subset of NK-T cells and weakly by dendritic cells and macrophages. CD4 plays an important role in the development of T cells and is required for mature T cells to function optimally.This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

Product Properties & Targets

Antibody Type
Host
Rat
Species Reactivity
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell membrane
Gene Name
CD4
Positive Control
Spleen, Thymus or Lymph Node.
Immunogen
Mouse CTL clone V4
Alternate Names
T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, CD4 (L3T4); CD4; CD4+ Lymphocyte deficiency, included; Leu3; Ly-4; Lymphocyte antigen CD4; p55; T cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
GK1.5
Chromosome Location
6 F2; 6 59.17 cM (Mouse)
Mol. Weight of Antigen
55kDa

Functions

  • Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.

Key References

  • Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  • Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  • Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  • Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  • Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  • Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  • Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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