Home >> Antibodies >> CD3e (T-Cell Marker)

CD3e (T-Cell Marker)

Recombinant Rabbit Monoclonal Antibody [Clone C3e/4653R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human tonsil stained with CD3e Recombinant Rabbit Monoclonal Antibody (CD3e/4653R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using C3e/4653R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Western Blot Analysis of human Jurkat cell lysate using CD3e Recombinant Rabbit Monoclonal Antibody (C3e/4653R).

Formalin-fixed, paraffin-embedded human tonsil stained with CD3e Recombinant Rabbit Monoclonal Antibody (CD3e/4653R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using C3e/4653R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Western Blot Analysis of human Jurkat cell lysate using CD3e Recombinant Rabbit Monoclonal Antibody (C3e/4653R).

Product Details

Synonyms

CD3 epsilon; CD3 TCR complex; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex

Positive Control

Human tonsil, spleen or lymph node. Human peripheral blood or Jurkat cells.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human CD3e fragment (around aa100-200) (exact sequence is proprietary)

Cellular Localization

Cell Surface. Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

20-25kDa

Specificity & Comments

Cluster of differentiation 3 (CD3) is a multimeric protein complex, known historically as the T3 complex, and is composed of four distinct polypeptide chains; epsilon ( ), gamma (), delta ( �L) and zeta ( ��), that assemble and function as three pairs of dimers ( , �L, �� ). The CD3 complex serves as a T cell co-receptor that associates noncovalently with the T cell receptor (TCR).CD3 antigen appearance at all stages of T cell development, and makes it an ideal T cell marker for both the detection of normal T cells and T cell neoplasms (lymphomas and leukemias).