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Recombinant CD3e (T-Cell Marker) Antibody [C3e/4652R]

In Stock
Catalog Number Formulation Size Price
916-RBM22-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
916-RBM22-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
916-RBM22-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD3e (T-Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human lymph node stained with CD3e Recombinant Rabbit Monoclonal Antibody (C3e/4652R).

Recombinant CD3e (T-Cell Marker) Antibody - Image 2

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using C3e/4652R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant CD3e (T-Cell Marker) Antibody - Image 3

IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong cell surface staining using C3e/4652R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant CD3e (T-Cell Marker) Antibody - Image 4

Western blot analysis of Jurkat cell lysate using CD3e Recombinant Rabbit Monoclonal Antibody (C3e/4652R).

CD3e Antibody in SDS-PAGE

CD3e Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD3e Recombinant Rabbit Monoclonal Antibody(CD3e/4652R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
ELISA, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Hematopoietic Stem Cells, PD-1 blockade immunotherapy
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane
Gene Name
CD3E
Positive Control
Human peripheral blood. Jurkat cells. Tonsil or lymph node.
Immunogen
Synthetic peptide from human CD3e protein (around aa 20-40) (exact sequence is proprietary)
Alternate Names
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3 epsilon; CD3 TCR complex; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex

Database Links

Entrez Gene ID
916
SwissProt
P07766

Additional Information

Clone
C3e/4652R
Chromosome Location
11q23.3
Mol. Weight of Antigen
20kDa

Related Antibodies

View All CD3E Antibodies

Functions

  • Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).

Key References

  • Beverley PC, Callard RE. Eur J Immunol. 1981; 11(4):329-334.
  • Mason DY et. al. Journal of Clinical Pathology, 1989, 42(11):1194-200.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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