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Home >> Antibodies >> CD100 (Semaphorin-4D)

CD100 (Semaphorin-4D)

Mouse Monoclonal Antibody [Clone 133-1C6]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00

Product Details

Synonyms

COLL4; Collapsin4; GR3; M sema G; MSEMA; SEMA4D; Semacl2; SemaH; SEMAJ; Semaphorin C like 2; Semaphorin H; Semaphorin J; Semaphorin-4D; Semcl2

Positive Control

Daudi, Raji, HUT-78, Kg1a, U937 and human lymphocytes. Human tonsils and lymph nodes.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

PHA stimulated human peripheral blood lymphocytes.

Cellular Localization

Cell surface

Species Reactivity

Human. Baboon. Does not react with Horse.

Host / Ig Isotype

Mouse / IgM, kappa

Mol. Weight of Antigen

50kDa (Monomer)

Specificity & Comments

Recognizes a homodimeric protein comprised of 50kDa subunits, identified as CD100 (Workshop VI; Code N-L026). It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Schlossman SF et al. (eds) Leucocyte Typing V Oxford University Press, Oxford. 1995.
  2. Kishimoto T et al. (eds) Leukocyte Typing VI, Garland Publishing, New York, 1997.
  3. Dorfman DM et al. The leukocyte semaphorin CD100 is expressed in most T-cell, but few B-cell, non-Hodgkin's lymphomas. Am J Pathol 1998, 153(1):255-262.
  4. Delaire S et al. CD100 is a leukocyte semaphorin. Cell Mol Life Sci 1998, 54(11):1265-1276.
  5. Herold C et al. CD100 is associated with CD45 at the surface of human T lymphocytes. Role in T cell homotypic adhesion. J Immunol 1996, 157(12):5262-5268.
  6. Elhabazi A et al. The human semaphorin-like leukocyte cell surface molecule CD100 associates with a serine kinase activity. J Biol Chem 1997, 272(38):23515-23520.
  7. Hall KT et al. Human CD100, a novel leukocyte semaphorin that promotes B-cell aggregation and differentiation. Proc Natl Acad Sci USA 1996, 93(21):11780-11785

Bioinformatics

  •  Entrez Gene ID: 10507
  •  Gene Symbol: SEMA4D
  •  SwissProt: Q92854
  •  Chromosome Location: 9q22.2
  •  Unigene: 494406
NBT00932