Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

Printable Datasheet

Catenin, alpha-1 (CTNNA1) Antibody [1G5]

In Stock
Catalog Number Formulation Size Price
1495-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1495-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1495-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Western Blot of A431 and MCF-7 cell lysate using Catenin, alpha-1 Mouse Monoclonal Antibody (1G5).

Western Blot of A431 and MCF-7 cell lysate using Catenin, alpha-1 Mouse Monoclonal Antibody (1G5).

SDS-PAGE Analysis of Purified Catenin, alpha-1 Mouse Monoclonal Antibody (1G5).

SDS-PAGE Analysis of Purified Catenin, alpha-1 Mouse Monoclonal Antibody (1G5).

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of 102kDa, identified as Catenin, alpha-1. Catenins comprise a large family of Ca2+-dependent, homotypic cell-cell adhesion molecules that play important roles in development, epithelial cell polarity and tumor progression. Alpha-catenin is a key regulator of actin dynamics in cell-cell adhesion. During cell-cell adhesion, -catenin and links the cadherins to actin associated with the cytoskeleton. Alpha-catenin also regulates the beta-catenin signaling in various cells. It displays the tumor suppressor activity and is found to be down regulated in some forms of breast cancer.'

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human
Research Areas
Autophagy, Cardiovascular, Developmental Biology, BBB VCAM-1 Signaling, Ovarian Cancer, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Adherens junction, Cell junction, Cell membrane, Cytoplasm, Cytoskeleton
Gene Name
CTNNA1
Positive Control
A431 or MCF-7 cells., HeLa
Immunogen
Human alpha catenin fusion protein
Alternate Names
Catenin alpha-1, Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-REN-13, Alpha E-catenin; Cadherin-associated protein; CAP102; Catenin alpha-1; CTNNA1; Renal carcinoma antigen NY-REN-13

Database Links

Entrez Gene ID
1495
SwissProt
P35221

Additional Information

Clone
1G5
Chromosome Location
5q31.2
Mol. Weight of Antigen
102kDa

Related Antibodies

View All CTNNA1 Antibodies

Functions

  • Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity). May play a crucial role in cell differentiation.

Key References

  • Johnson, KR; Lewis, JE; Li, D; Wahl, J; Soler, AP; Knudsen, KA; Wheelock, MJ. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 1993, 207: 252-60
  • Nieman, MT; Kim, JB; Johnson, KR; Wheelock, MJ. Mechanism of extracellular domain-deleted dominant negative cadherins. J Cell Science 1999, 112: 1621-32

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Catenin, alpha-1 (CTNNA1) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales