Western Blot of A431 and MCF-7 cell lysate using Catenin, alpha-1 Mouse Monoclonal Antibody (1G5).

SDS-PAGE Analysis Purified Catenin, alpha-1 Mouse Monoclonal Antibody (1G5).

Printable Datasheet

Catenin, alpha-1 (CTNNA1) [1G5]

Name: Catenin, alpha-1 (CTNNA1)
SKU: 1495-MSM1
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

Recognizes a protein of 102kDa, identified as Catenin, alpha-1. Catenins comprise a large family of Ca2+-dependent, homotypic cell-cell adhesion molecules that play important roles in development, epithelial cell polarity and tumor progression. Alpha-catenin is a key regulator of actin dynamics in cell-cell adhesion. During cell-cell adhesion, -catenin and links the cadherins to actin associated with the cytoskeleton. Alpha-catenin also regulates the beta-catenin signaling in various cells. It displays the tumor suppressor activity and is found to be down regulated in some forms of breast cancer.'

Product Properties & Targets

Host

Mouse

Species Reactivity

Human

Research Areas

Autophagy, Cardiovascular

Isotype

IgG1

Cellular Localization

Adherens junction, Cell junction, Cell membrane, Cytoplasm, Cytoskeleton

Light Chain

kappa

Gene Name

CTNNA1

Positive Control

A431 or MCF-7 cells., HeLa

Immunogen

Human alpha catenin fusion protein

Alternate Names

Catenin alpha-1, Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-REN-13, Alpha E-catenin; Cadherin-associated protein; CAP102; Catenin alpha-1; CTNNA1; Renal carcinoma antigen NY-REN-13

Database Links

Entrez Gene ID

1495

Unigene

445981

SwissProt

P35221

Additional Information

Clone

1G5

Human Gene Symbol

CTNNA1

Chromosome Location

5q31.2

Mol. Weight of Antigen

102kDa

Functions

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity). May play a crucial role in cell differentiation.

Known Applications & Suggested Dilutions

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Key References

Johnson, KR; Lewis, JE; Li, D; Wahl, J; Soler, AP; Knudsen, KA; Wheelock, MJ. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 1993, 207: 252-60
Nieman, MT; Kim, JB; Johnson, KR; Wheelock, MJ. Mechanism of extracellular domain-deleted dominant negative cadherins. J Cell Science 1999, 112: 1621-32

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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