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Home >> Antibodies >> Calcitonin

Calcitonin

Mouse Monoclonal Antibody [Clone CALCA/3310]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human thyroid stained with Calcitonin Mouse Monoclonal Antibody (CALCA/3310). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

Formalin-fixed, paraffin-embedded human thyroid stained with Calcitonin Mouse Monoclonal Antibody (CALCA/3310). Inset: PBS instead of primary antibody; secondary only negative control.

img

Formalin-fixed, paraffin-embedded human thyroid stained with Calcitonin Mouse Monoclonal Antibody (CALCA/3310). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using Calcitonin Mouse Monoclonal Antibody (CALCA/3310).

Product Details

Synonyms

Alpha type CGRP; CALC1; calcitonin gene related peptide 1; CT; katacalcin (KC)

Positive Control

Human thyroid.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1:10-1:20 for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human Calcitonin protein (around aa 3-116) (exact sequence is proprietary)

Cellular Localization

Cytoplasm. Secreted

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

14.5kDa

Specificity & Comments

Calcitonin is a 32 amino acid polypeptide hormone that preserves skeletal integrity and reduces blood calcium levels by decreasing osteoclast activity in bones, calcium and phosphate reabsorption by kidney tubules and calcium absorption by the intestines. The secretion of Calcitonin from the thyroid is regulated in part by estrogen, which increases Calcitonin mRNA levels. The Calcitonin gene, CALCA, undergoes tissue-specific RNA alternative splicing, resulting in the production of different mRNA transcripts. One transcript encodes procalcitonin as well as both calcium-lowering processed active polypeptides, Calcitonin and katacalcin. An alternative transcript of CALCA encodes the precursor for the neuropeptide referred to as Calcitonin generelated peptide 1, also designated CGRP1 or -CGRP. CGRP is a widely distributed vasodilatory peptide. Calcitonin and katacalcin are produced primarily in the thyroid, while CGRP is produced in neuronal cells. A second CGRP related gene, CALCB, thought to be derived from an gene duplication event, has been identified in mouse, rat and human. Unlike CALCA, CALCB is not subject to alternative splicing and encodes a single transcript designated CGRP2 or -CGRP. Mature CGRP1 and CGRP2 share significant sequence identity at the protein level differing by only 1-3 amino acid residues, depending on the species.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Le Moullec, J.M., et al. 1984. The complete sequence of human preprocalcitonin. FEBS Lett. 167: 93-97.

Bioinformatics

  •  Entrez Gene ID: 796
  •  Gene Symbol: CALCA
  •  SwissProt: P01258
  •  Chromosome Location: 11p15.1
  •  Unigene: 37058