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Home >> Antibodies >> Beta-2 Microglobulin (Renal Failure & Tumor Marker)

Beta-2 Microglobulin (Renal Failure & Tumor Marker)

Mouse Monoclonal Antibody [Clone B2M/961]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human melanoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human gastric carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human colon carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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SDS-PAGE Analysis Purified Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of THP-1 and Raji cell lysates using Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

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Immunofluorescence Analysis of HeLa cells labeling with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961) followed by goat anti-mouse IgG-CF488 (green). nuclei stained with RedDot (red).

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Flow Cytometric Analysis of human HeLa cells. Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Product Details

Synonyms

B2M; Beta 2 microglobin; Beta 2 microglobulin; Beta chain of MHC class I molecules; Beta-2-microglobulin form pI 5.3; Hdcma22p

Positive Control

THP-1, Raji, HL-60 or HeLa cells. Carcinoma of Stomach, Cervix, Endometrial, Kidney or Colon.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-4ug/ml)
Western Blot (0.5-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Full length recombinant human B2M protein

Cellular Localization

Cell Surface and Extracellular (Secreted)

Species Reactivity

Human and non-human primates.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

12kDa

Specificity & Comments

Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Josson, S., et al. 2011. β2-Microglobulin induces epithelial to mesenchymal transition and confers cancer lethality and bone metastasis in human cancer cells. Cancer Res. 71: 2600-2610.

Bioinformatics

  •  Entrez Gene ID: 567
  •  Gene Symbol: B2M
  •  SwissProt: P61769
  •  Chromosome Location: 15q21-q22.2
  •  Unigene: 534255