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Home >> Antibodies >> Apolipoprotein B / APOB

Apolipoprotein B / APOB

Mouse Monoclonal Antibody [Clone APOB/4332]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human liver stained with Apolipoprotein B Mouse Monoclonal Antibody (APOB/4332).

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SDS-PAGE Analysis Purified Apolipoprotein B Mouse Monoclonal Antibody (APOB/4332). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Apolipoprotein B Mouse Monoclonal Antibody (APOB/4332).

Product Details

Synonyms

ApoB 100; ApoB 48; Apolipoprotein B (including Ag(x) antigen); Apolipoprotein B 100; Apolipoprotein B 48; Apolipoprotein B; FLDB; LDLCQ4

Positive Control

Human liver or kidney tissue.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human recombinant APOB protein fragment (around aa 592-689) (exact sequence is proprietary)

Cellular Localization

Cytoplasm. Secreted.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

512kDa

Specificity & Comments

Post-transcriptional editing of apolipoprotein B (apoB) mRNA is regulated byAPOBEC1 (also designated human (or rat) small intestinal apolipoprotein B mRNA editing protein, HEPR or REPR) in hepatic cells to achieve a steady state proportion of edited and unedited RNA molecules. Two forms of apoB are known to circulate in the plasma of mammals. ApoB-100 is a protein primarily synthesized in the liver as a structural component of very low density lipoprotein particles. A truncated form of apoB-100, apoB-48, is synthesized in the small intestine and contains the amino-terminal 2,152 amino acids of the larger protein. This organ-specific partitioning of apoB production is the result of RNA editing of a common apoB gene.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Manthey, K.C., et al. 2005. Riboflavin deficiency impairs oxidative folding and secretion of apolipoprotein B-100 in Hep G2 cells, triggering stress response systems. J. Nutr. 135: 978-982.

Bioinformatics

  •  Entrez Gene ID: 338
  •  Gene Symbol: APOB
  •  SwissProt: P04114
  •  Chromosome Location: 2p24.1
  •  Unigene: 120759