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Apolipoprotein B /APOB Antibody [APOB/3300]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
338-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
338-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
338-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Post-transcriptional editing of apolipoprotein B (apoB) mRNA is regulated byAPOBEC1 (also designated human (or rat) small intestinal apolipoprotein BmRNA editing protein, HEPR or REPR) in hepatic cells to achieve a steadystate proportion of edited and unedited RNA molecules. Two forms of apoBare known to circulate in the plasma of mammals. ApoB-100 is a proteinprimarily synthesized in the liver as a structural component of very low densitylipoprotein particles. A truncated form of apoB-100, apoB-48, is synthesizedin the small intestine and contains the amino-terminal 2,152 amino acids ofthe larger protein. This organ-specific partitioning of apoB production is theresultof RNA editing of a common apoB gene.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cytoplasm, Lipid droplet, Secreted
Gene Name
Positive Control
Human liver or kidney tissue.
Immunogen
Human recombinant APOB protein fragment (around aa592-689) (exact sequence is proprietary)
Alternate Names
Apolipoprotein B-100, ApoB 100; ApoB 48; Apolipoprotein B (including Ag(x) antigen); Apolipoprotein B 100; Apolipoprotein B 48; Apolipoprotein B; FLDB; LDLCQ4

Database Links

Entrez Gene ID
338
SwissProt

Additional Information

Clone
APOB/3300
Chromosome Location
2p24.1
Mol. Weight of Antigen
512kDa

Functions

  • Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Key References

  • Manthey, K.C., et al. 2005. Riboflavin deficiency impairs oxidative foldingand secretion of apolipoprotein B-100 in Hep G2 cells, triggering stressresponse systems. J. Nutr. 135: 978-982.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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