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Home >> Antibodies >> APC / Adenomatous Polyposis Coli / FAP (Tumor Suppressor)

APC / Adenomatous Polyposis Coli / FAP (Tumor Suppressor)

Mouse Monoclonal Antibody [Clone ALi 12-28]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified APC Mouse Monoclonal Antibody (ALi 12-28). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Adenomatous Polyposis Coli; CC1; Deleted in polyposis 2.5 (DP2.5); DP2; DP3; FAP; FPC; GS; Protein APC

Positive Control

COLO 320DM whole cell lysate. HCT116 cells. Tonsil, placenta, breast or colon.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IF, Western Blot         More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human APC protein (around aa 1-433)

Cellular Localization

Cell membrane and cytoplasm.

Species Reactivity

Human. Does not react with Mouse.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

66kDa

Specificity & Comments

The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. The cloning of the APC gene revealed a ubiquitously expressed protein, 2,843 amino acids in length, which is frequently mutated in patients suffering from FAP and GS. APC has been found to be associated with structural components of intracellular junctions. Functions as a tumor suppressor, promoting rapid degradation of CTNNB1 and is a negative regulator of Wnt signaling. Also plays a role in HGF-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Sunkel, C.E. and Glover, D.M. 1988. J. Cell Sci. 89: 25-38.

Bioinformatics

  •  Entrez Gene ID: 324
  •  Gene Symbol: APC
  •  SwissProt: P25054
  •  Chromosome Location: 5q22.2
  •  Unigene: 158932
NBT03076