2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Alkaline Phosphatase (Tissue-Nonspecific)

Alkaline Phosphatase (Tissue-Nonspecific)

Mouse Monoclonal Antibody [Clone SPM372]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00

Product Details

Synonyms

Alkaline phosphatase, germ cell; Alkaline phosphatase, testicular and thymus; Alkaline phosphatase liver/bone/kidney; Alkaline phosphatase, tissue-nonspecific; ALPG; ALPL; Germ cell alkaline phosphatase; Testicular and thymus alkaline phosphatase; Tissue non-specific alkaline phosphatase; Tissue nonspecific ALP (TNAP or TNSALP)

Positive Control

Intestine.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-Fr         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Frozen) (1-2ug/ml for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Immunogen

Bovine intestinal alkaline phosphatase

Cellular Localization

Cell Surface

Species Reactivity

Human. Cow.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

55kDa

Specificity & Comments

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Moss, D.W. 1987. Diagnostic aspects of alkaline phosphatase and its isoenzymes. Clin. Biochem. 20: 225-230.
  2. Griffin, C.A., et al. 1987. Human placental and intestinal alkaline phosphatase genes map to 2q34-q37. Am. J. Hum. Genet. 41: 1025-1034.

Bioinformatics

  •  Entrez Gene ID: 249
  •  Gene Symbol: ALPL
  •  SwissProt: P05186
  •  Chromosome Location: 1p36.12
  •  Unigene: 75431
NBT00141