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Home >> Antibodies >> Actin alpha 1, Skeletal Muscle

Actin alpha 1, Skeletal Muscle

Mouse Monoclonal Antibody [Clone ACTA1/360]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human skeletal muscle stained with Skeletal Muscle Actin Mouse Monoclonal (ACTA1/360). Inset: PBS instead of primary antibody; secondary only negative control.

img

Formalin-fixed, paraffin-embedded human skeletal muscle stained with Skeletal Muscle Actin Mouse Monoclonal (ACTA1/360). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. AB, 5min.

Product Details

Synonyms

a actin; ACTA; ACTA1; Actin, alpha skeletal muscle; ACTS_HUMAN; Alpha actin 1; Alpha-actin-1; ASMA; MPFD; Skeletal muscle alpha actin 1

Positive Control

Skeletal muscle.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human ACTA1 protein

Cellular Localization

Cytoplasm.

Species Reactivity

Human.Predicted to show a broad reactivity.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

43kDa

Specificity & Comments

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas b and g are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Yang K,et al. Identification of SERPINE1, PLAU and ACTA1 as biomarkers of head and neck squamous cell carcinoma based on integrated bioinformatics analysis. Int J Clin Oncol. 2019;24(9):1030-1041.PMID: 30937621

Bioinformatics

  •  Entrez Gene ID: 58
  •  Gene Symbol: ACTA1
  •  SwissProt: P68133
  •  Chromosome Location: 1q42.13
  •  Unigene: 1288