| Catalog Number: | 58-MSM1 |
|---|---|
| Reactivity: | Guinea Pig, Hamster, Human, Mouse, Rat |
| Gene: | |
| Host: |
Trial Size Available
Carrier-Free Available
Formalin-fixed, paraffin-embedded human skeletal muscle stained with Skeletal Muscle Actin Mouse Monoclonal (ACTA1/360). Inset: PBS instead of primary antibody; secondary only negative control.
Formalin-fixed, paraffin-embedded human skeletal muscle stained with Skeletal Muscle Actin Mouse Monoclonal (ACTA1/360). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. AB, 5min.
Western Blot Analysis of HeLa, T47D lysate using Actin alpha 1, Skeletal Muscle Mouse Monoclonal Antibody (ACTA1/360)
SDS-PAGE Analysis Purified ACTA1 Mouse Monoclonal Antibody (ACTA1/360). Confirmation of Purity and Integrity of Antibody.
Western blot analysis of Human Intestine, Mouse Intestine, Rat Intestine, Hamster Intestine, Guinea pig Intestine, Human skeletal muscle, Mouse skeletal muscle, Rat skeletal muscle, Hamster skeletal muscle and Guinea pig skeletal muscle lysates using Actin alpha 1, Skeletal Muscle Mouse Monoclonal Antibody (ACTA1/360)
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas b and g are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.
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