SDS-PAGE Analysis Purified TDRKH Mouse Monoclonal Antibody (PCRP-TDRKH-1H2). Confirmation of Purity and Integrity of Antibody.

Immunofluorescence analysis of PFA-fixed HeLa cells. TDRKH Mouse Monoclonal Antibody (PCRP-TDRKH-1H2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow cytometric analysis of PFA-fixed HeLa cells. TDRKH Mouse Monoclonal Antibody (PCRP-TDRKH-1H2) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained. TDRKH Mouse Monoclonal Antibody (PCRP-TDRKH-1H2) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Analysis of Protein Array containing more than 19,000 full-length human proteins using TDRKH-Monospecific Mouse Monoclonal Antibody (PCRP-TDRKH-1H2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Printable Datasheet

TDRKH [PCRP-TDRKH-1H2]

Name: TDRKH
SKU: 11022-MSM1
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.

Product Properties & Targets

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Species Reactivity

Human

Isotype

IgG2b

Cellular Localization

Cytoplasm, Mitochondrion

Light Chain

Kappa

Gene Name

TDRKH

Positive Control

HeLa or MCF7 cells. Human testis or brain.

Immunogen

Recombinant full-length human TDRKH protein

Alternate Names

Tudor and KH domain-containing protein, Tudor domain-containing protein 2, 2700091C21Rik; Putative RNA binding protein; RGD1311196; RP11-98D18.8; TDRD2; TDRD7; Tdrkh; Tudor and KH domain containing; Tudor and KH domain-containing protein; tudor domain containing 2

Database Links

Entrez Gene ID

11022

Unigene

144439

SwissProt

Q9Y2W6

Additional Information

Clone

PCRP-TDRKH-1H2

Human Gene Symbol

TDRKH

Chromosome Location

1q21.3

Mol. Weight of Antigen

62.05kDa

Functions

Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.

Known Applications & Suggested Dilutions

Immunoprecipitation (1-2ug per 100-500ug of total protein)
Flow Cytometry (1-2ug/million cells)
,Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Blackshaw S, et al. Nature methods 15.5 (2018 May): 330-338.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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