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Recombinant TBXT / T-box transcription factor T / Brachyury Antibody [TBXT/7711R]

In Stock
Catalog Number Formulation Size Price
6862-RBM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6862-RBM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6862-RBM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The T-box gene family consists of members that share a unique DNA binding domain. The best characterized T-box (Tbx) gene, brachyury or T, encodes a transcription factor that plays an important role in early vertebrate development. Tbx genes are a family of developmental regulators with more than 20 members recently identified among invertebrates and vertebrates. Mutations in Tbx genes have been found to cause several human diseases. The understanding of functional mechanisms of Tbx products has come mainly from the prototypical T/brachyury protein, which is a transcription activator. The T-domain is a highly conserved DNA-binding motif originally defined in brachyury and characteristic of the Tbx family of transcription factors. The murine brachyury (T) gene is required in posterior mesoderm formation and axial development. Mutant embryos lacking T gene function are deficient in notochord differentiation and posterior mesoderm formation, but develop anterior mesoderm.

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus.
Gene Name
Positive Control
Human chordoma tissue.
Immunogen
Recombinant fragment (around aa 235-435) of human TBXT protein (exact sequence is proprietary)
Alternate Names
T-box transcription factor T, Brachyury protein, Protein T, BRAC_HUMAN; Brachyury homolog; Brachyury protein; Bry; MGC104817; Protein T; SAVA; T; T brachyury homolog; T brachyury transcription factor; T Protein; T, brachyury homolog (mouse); TFT; Transcription factor T

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
TBXT/7711R
Chromosome Location
6q27
Mol. Weight of Antigen
47kDa

Functions

  • Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates gene transcription when bound to such a site.

Key References

  • Kispert, A. and Herrmann, B.G. 1994. Immunohistochemical analysis of the brachyury protein in wild-type and mutant mouse embryos. Dev. Biol. 161: 179-193.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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