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Recombinant Steroidogenic Factor 1 (SF-1) Antibody [SF1/8000R]

In Stock
Catalog Number Formulation Size Price
2516-RBM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2516-RBM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2516-RBM11-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human testis stained with SF-1 Recombinant Rabbit Monoclonal Antibody (SF1/8000R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human testis stained with SF-1 Recombinant Rabbit Monoclonal Antibody (SF1/8000R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human adrenal cortex stained with SF-1 Recombinant Rabbit Monoclonal Antibody (SF1/8000R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human adrenal cortex stained with SF-1 Recombinant Rabbit Monoclonal Antibody (SF1/8000R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Steroidogenic Factor 1 (SF-1) is considered an orphan nuclear receptor that belongs to subfamily 5. It was found to be a regulator of steroidogenic enzyme gene expression. Oxysterols are suggested as its ligands. It is expressed in all steroidogenic tissues, including the adrenal cortex, testicular Sertoli cells, and Leydig cells, ovarian theca, hypothalamus, and anterior pituitary. SF-1 plays an important role in adrenal and gonadal development.SF-1 is highly valuable marker to determine the adrenocortical origin of an adrenal mass.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human, Rat
Research Areas
Cardiovascular, Developmental Biology, Nuclear Marker, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus.
Gene Name
NR5A1
Positive Control
Human testis or adrenal cortical carcinoma.
Immunogen
Recombinant fragment (around aa200-400) of human SF-1 protein (exact sequence is proprietary)
Alternate Names
Steroidogenic factor 1, Adrenal 4-binding protein, Fushi tarazu factor homolog 1, Nuclear receptor subfamily 5 group A member 1, Steroid hormone receptor Ad4BP, Adrenal 4 binding protein (AD4BP); ELP; Fushi tarazu factor homolog 1 (FTZ1); Nuclear receptor AdBP4; Nuclear receptor subfamily 5 group A member 1 (NR5A1); POF7; SPGF8; SRXY3; Steroid hormone receptor Ad4BP; Steroidogenic factor 1 (SF1); Steroidogenic factor 1 nuclear receptor; STF1

Database Links

Entrez Gene ID
2516
SwissProt
Q13285

Additional Information

Clone
SF1/8000R
Chromosome Location
9q33.3
Mol. Weight of Antigen
53kDa

Related Antibodies

View All NR5A1 Antibodies

Functions

  • Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues (PubMed:27378692). Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1 (PubMed:27378692). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3, 4)P2 and PI(3, 4, 5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.

Key References

  • Zhao C, Vinh TN, et al. Am J Surg Pathol. 2009; 33:354-66.
  • Sangoi AR, et al. AmJ Surg Pathol. 2011; 35:678-86.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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