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Recombinant NKX3.1 (Metastatic Prostate Adenocarcinoma Marker) Antibody [NKX3.1/4562R]

In Stock
Catalog Number Formulation Size Price
4824-RBM12-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4824-RBM12-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4824-RBM12-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human prostate stained with NKX3.1 Recombinant Rabbit Monoclonal Antibody (NKX3.1/4562R).

Formalin-fixed, paraffin-embedded human prostate stained with NKX3.1 Recombinant Rabbit Monoclonal Antibody (NKX3.1/4562R).

Formalin-fixed, paraffin-embedded human prostate stained with NKX3.1 Recombinant Rabbit Monoclonal Antibody (NKX3.1/4562R).

Formalin-fixed, paraffin-embedded human prostate stained with NKX3.1 Recombinant Rabbit Monoclonal Antibody (NKX3.1/4562R).

SDS-PAGE Analysis of Purified NKX3.1 Recombinant Rabbit Monoclonal Antibody (NKX3.1/4562R). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified NKX3.1 Recombinant Rabbit Monoclonal Antibody (NKX3.1/4562R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

NKX3.1 is a prostate specific gene encoding a transcription factor that plays an important role in normal prostate development and carcinogenesis. It is a prostatic tumor suppressor gene located on chromosome 8p21.2, which frequently undergoes a loss of heterozygosity. NKX3.1 expression is highly restricted in prostate epithelial cells and therefore can be used as a diagnostic biomarker for prostate cancer and other metastatic lesions of prostatic origin. Furthermore, NKX3.1 shows better sensitivity than Prostate Specific Antigen (PSA) for identifying metastatic prostatic adenocarcinoma. This suggests that immunohistochemical staining of NKX3.1, along with other prostate-restricted markers, may be valuable for the definitive determination of prostatic origin in poorly differentiated metastatic carcinomas.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
ELISA, Immunohistochemistry
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus
Gene Name
NKX3-1
Positive Control
Highly expressed in the prostate and at a lower level in the testis.
Immunogen
Recombinant fragment (around aa 92-224) of human NKX3.1 protein (exact sequence is proprietary)
Alternate Names
Homeobox protein Nkx-3.1, Homeobox protein NK-3 homolog A, NK3 Homeobox 1; Homeobox Protein NK-3 Homolog A; NKX3-1; NKX3A; NK3 Transcription Factor Related, Locus 1 (Drosophila); NK3 Transcription Factor Related, Locus 1; NK Homeobox (Drosophila), Family 3, A; NK3 Transcription Factor Homolog A; NK Homeobox, Family 3, A; Homeobox Protein Nkx-3.1; BAPX2; NKX3

Database Links

Entrez Gene ID
4824
SwissProt
Q99801

Additional Information

Clone
NKX3.1/4562R
Chromosome Location
8p21.2
Mol. Weight of Antigen
35kDa

Related Antibodies

View All NKX3-1 Antibodies

Functions

  • Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.

Key References

  • Zhang P., et al. Mol Biol Rep.2010 Mar;37(3):1505-12.
  • Gurel B., et al. Am J Surg Pathol.2010 Aug;34(8):1097-105.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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