Formalin-fixed, paraffin-embedded human prostate stained with NKX3.1 Recombinant Rabbit Monoclonal Antibody (NKX3.1/4562R).

SDS-PAGE Analysis Purified NKX3.1 Recombinant Rabbit Monoclonal Antibody (NKX3.1/4562R). Confirmation of Purity and Integrity of Antibody.

NKX3.1 (Metastatic Prostate Adenocarcinoma Marker) [NKX3.1/4562R]

Name: NKX3.1 (Metastatic Prostate Adenocarcinoma Marker)
SKU: 4824-RBM12
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

NKX3.1 is a prostate specific gene encoding a transcription factor that plays an important role in normal prostate development and carcinogenesis. It is a prostatic tumor suppressor gene located on chromosome 8p21.2, which frequently undergoes a loss of heterozygosity. NKX3.1 expression is highly restricted in prostate epithelial cells and therefore can be used as a diagnostic biomarker for prostate cancer and other metastatic lesions of prostatic origin. Furthermore, NKX3.1 shows better sensitivity than Prostate Specific Antigen (PSA) for identifying metastatic prostatic adenocarcinoma. This suggests that immunohistochemical staining of NKX3.1, along with other prostate-restricted markers, may be valuable for the definitive determination of prostatic origin in poorly differentiated metastatic carcinomas.

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Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Nucleus

Gene Name

NKX3-1

Mol. Weight of Antigen

35kDa

Immunogen

Recombinant fragment (around aa 92-224) of human NKX3.1 protein (exact sequence is proprietary)

Database Links

Entrez Gene ID

4824

Unigene

55999

SwissProt

Q99801

Additional Information

Clone

NKX3.1/4562R

Human Gene Symbol

NKX3-1

Chromosome Location

8p21.2

Known Applications & Suggested Dilutions

ELISA (For coating, order Ab without BSA)
,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Zhang P., et al. Mol Biol Rep.2010 Mar;37(3):1505-12.
Gurel B., et al. Am J Surg Pathol.2010 Aug;34(8):1097-105.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.