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CD84 / SLAMF5 Antibody [152-1D5]

Additional information

Catalog Number: 8832-MSM1
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
8832-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
8832-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
8832-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD84 / SLAMF5 Antibody

SDS-PAGE Analysis of Purified CD84 Mouse Monoclonal Antibody (152-1D5). Confirmation of Purity and Integrity of Antibody.

CD84 / SLAMF5 Antibody - Image 2

Flow Cytometric Analysis of PFA-fixed MOLT-4 cells. CD84 Mouse Monoclonal Antibody (152-1D5) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

  • Flow
  • IF
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human
Clone
152-1D5
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell membrane
Gene Name
CD84
Positive Control
HL60, HMC-1, Molt-4, Raji, THP-1, HEL or NAMALWA cells. Human platelets and macrophages. Human tonsil.
Immunogen
Spleen cells of a patient with hairy cell leukemia
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Can mediate natural killer (NK) cell cytotoxicity dependent on SH2D1A and SH2D1B. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seem be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A-dependent pathway. May serve as a marker for hematopoietic progenitor cells. Required for a prolonged T-cell:B-cell contact, optimal T follicular helper function, and germinal center formation. In germinal centers involved in maintaining B-cell tolerance and in preventing autoimmunity. In mast cells negatively regulates high affinity immunoglobulin epsilon receptor signaling; independent of SH2D1A and SH2D1B but implicating FES and PTPN6/SHP-1. In macrophages enhances LPS-induced MAPK phosphorylation and NF-kappaB activation and modulates LPS-induced cytokine secretion; involving ITSM 2. Positively regulates macroautophagy in primary dendritic cells via stabilization of IRF8; inhibits TRIM21-mediated proteasomal degradation of IRF8.
Key References
  • Schlossman SF et al. (eds) Leukocyte Typing V. p.699-700, Oxford University Press, Oxford, 1995.
  • Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, Inc, New York, 1997
  • de la Fuente MA et al. CD84 leukocyte antigen is a new member of the Ig superfamily. Blood 1997, 90(6):2398-2405.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
8832
SwissProt
Q9UIB8
Chromosome Location
1q24
Mol. Weight of Antigen
74kDa
Alternate Names
SLAM family member 5, Cell surface antigen MAX.3, Hly9-beta, Leukocyte differentiation antigen CD84, Signaling lymphocytic activation molecule 5, CD84, Cell surface antigen MAX.3; hCD84; Hly9-beta; Leucocyte differentiation antigen CD84; LY9B; Signaling lymphocytic activation molecule 5 (SLAMF5); SLAM family member 5

Summary

Recognizes a protein of 74kDa, identified as CD84 (Workshop V; Code A085, C057). It is expressed on mature B cells and on B-cell lines, including pre-B-cell lines, but not on plasma cell lines. Immunohistochemical studies demonstrated that CD84 strongly expressed on tissues macrophages. CD84 is also highly expressed on platelets and, at low levels on peripheral blood T cells. It is a highly N-glycosylated protein and belongs to immunoglobulin superfamily. It may play a role in leukocyte activation.

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