Western blot analysis of human heart tissue lysate using N-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH2/6857R).

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with N-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH2/6857R).

Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH2/6857R).

Printable Datasheet

Recombinant N-Cadherin / Cadherin-2 / CD325 (NCAD) [CDH2/6857R]

Name: N-Cadherin / Cadherin-2 / CD325 (NCAD)
SKU: 1000-RBM7
Availability: InStock

Primary Antibodies Monoclonals Recombinants Recombinant Rabbit Monoclonals Rabbit Monoclonals

Summary

Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells.Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

Product Properties & Targets

Host

Rabbit

Applications

Immunohistochemistry, Western Blot

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Cell junction, Cell membrane, Cell surface, Sarcolemma

Light Chain

Kappa

Gene Name

CDH2

Positive Control

Human heart, pancreas or cerebral cortex (IHC).

Immunogen

Recombinant full-length human CDH2 protein

Alternate Names

Cadherin-2, CDw325, Neural cadherin, Cadherin-2 N cadherin neuronal; Cadherin-2 type 1; Cadherin-2; Calcium dependent adhesion protein neuronal; CD325; CDH2; CDHN; CDw325; N-Cadherin; NCAD

Database Links

Entrez Gene ID

1000

Unigene

464829

SwissProt

P19022

Additional Information

Clone

CDH2/6857R

Human Gene Symbol

CDH2

Chromosome Location

18q11.2

Mol. Weight of Antigen

130-140kDa

Functions

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

Known Applications & Suggested Dilutions

Western Blot (1-2ug/ml)
,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Wahl, JK III et al.N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane. J Biol Chem. 2003, 278(19):17269-76.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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