MSH6 (DNA Mismatch Repair Protein) [MSH6/6654R]

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Summary

MSH6 is a mismatch repair protein which is deficient in a high proportion of patients with microsatellite instability (MSI-H). It has been suggested that the deficiencies in DNA mismatch repair protein(s) can be seen in some malignancies such as hereditary nonpolyposis colorectal cancer (HNPCC) and endometrial cancer. MSH6 expressed in all proliferating cells participate in repair of base-base mismatch, that occur during DNA replication. Loss of MSH6 expression leads to an accumulation of DNA replication errors in the proliferating cells, particularly in areas of the genome with short repetitive nucleotide sequences, a phenomenon known as microsatellite instability (MSI). MSH6 always used as panel with MLH1, MSH2, PMS2, and may be useful to aid in identifying the most probable gene responsiblefor the MSI.

Product Properties & Targets

Host
Species Reactivity
Isotype
IgG
Cellular Localization
Gene Name
MSH6
Mol. Weight of Antigen
153kDa
Immunogen
Recombinant fragment (around aa 1-100) of human MSH6 protein (exact sequence is proprietary)

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
MSH6/6654R
Human Gene Symbol
MSH6
Chromosome Location
2p16.3

Known Applications & Suggested Dilutions

  • ELISA (For coating, order Ab without BSA)
  • Western Blot (1-2ug/ml)
  • ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determin

Key References

  • Blackwell LJ., et al. J Biol Chem. 1998 Nov 27;273(48):32055-62.
  • Iaccarino I., et al. EMBO J. 1998 May 1;17(9):2677-86.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Recombinant mouse monoclonal antibodies and recombinant rabbit monoclonals to support your research

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