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Recombinant MSH6 (DNA Mismatch Repair Protein) Antibody [MSH6/13194R]

Additional information

Catalog Number: 2956-RBM24
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

download data sheet
MSH6 Antibody in Immunohistochemistry (IHC (P))

MSH6 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human colon stained with MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/13194R). Inset: PBS instead of primary antibody; secondary only negative control.

MSH6 Antibody in Western Blot (WB)

MSH6 Antibody in Western Blot (WB)

Western Blot Analysis of DU 145 lysate using MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/13194R).

MSH6 Antibody in SDS-PAGE

MSH6 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified DNA mismatch repair protein Msh6 Recombinant Rabbit Monoclonal Antibody (MSH6/13194R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
In Stock
Catalog Number Formulation Availability Size Price
2956-RBM24-P0
Purified Ab with BSA and Azide at 200ug/ml
Trial Size
In Stock
20ug
$259.00
Trial Size Available In-Stock
2956-RBM24-P1
Purified Ab with BSA and Azide at 200ug/ml
In Stock
100ug
$559.00
In-Stock
2956-RBM24-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
Carrier-Free
In Stock
100ug
$559.00
Carrier-Free Available In-Stock
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
MSH6/13194R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Chromosome, Nucleus
Gene Name
MSH6
Positive Control
DU 145,Human colon carcinoma or tonsil.
Immunogen
Recombinant fragment (around aa1-13) of the human MSH6 protein (exact sequence is proprietary)
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2956
SwissProt
P52701
Chromosome Location
2p16.3
Mol. Weight of Antigen
152.78kDa
Alternate Names
DNA mismatch repair protein Msh6, GTBP|GTMBP|HNPCC5|HSAP|LYNCH5|MMRCS3|MSH-6|p160

Summary

MSH6 is a mismatch repair protein which is deficient in a high proportion of patients with microsatellite instability (MSI-H). It has been suggested that the deficiencies in DNA mismatch repair protein(s) can be seen in some malignancies such as hereditary nonpolyposis colorectal cancer (HNPCC) and endometrial cancer. MSH6 expressed in all proliferating cells participate in repair of base-base mismatch, that occur during DNA replication. Loss of MSH6 expression leads to an accumulation of DNA replication errors in the proliferating cells, particularly in areas of the genome with short repetitive nucleotide sequences, a phenomenon known as microsatellite instability (MSI). MSH6 always used as panel with MLH1, MSH2, PMS2, and may be useful to aid in identifying the most probable gene responsiblefor the MSI.

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