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MICA Antibody [MICA/4443]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
100507436-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
100507436-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
100507436-MSM3-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

MICA and MICB are stress-induced antigens that are related to major histocompatibility complex (MHC) class I molecules. MICA and MICB are frequently expressed in epithelial tumors. These highly glycosylated cell surface proteins are stably expressed without conventional class I peptide ligands or association with β-2-microglobulin. The expression is induced on proliferating or heat shock-stressed epithelial cells. MICA and MICB are broadly recognized by intestinal epithelial Vδ1 γδ T cells expressing variable TCRs, suggesting that these antigens may play a central role in the signaling of cellular distress to evoke immune responses in the intestinal epithelium.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell membrane, Cytoplasm
Gene Name
Positive Control
breast or prostate., HeLa or MCF-7 cells. Human kidney
Immunogen
Recombinant fragment (around aa1-200) of human MICA (exact sequence is proprietary)
Alternate Names
MHC class I polypeptide-related sequence A, HLA class I antigen; MHC class I chain related gene A protein antibody; MHC class I chain related protein A; MHC class I chain related protein A HLA B HLA C; MHC class I polypeptide related sequence A; MHC class I polypeptide-related sequence A; MHC class I related protein; MIC A; MIC-A; micA; PERB11.1; Stress inducible class I homolog

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
MICA/4443
Chromosome Location
6p21.33
Mol. Weight of Antigen
92kDa

Functions

  • Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.

Key References

  • Bedel, R., et al. 2011. Novel role for STAT3 in transcriptional regulation of NK immune cell targeting receptor MICA on cancer cells. Cancer Res. 71:1615-1626.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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