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Human Nuclear Antigen (HNA) (Human Cell Marker) Antibody [235-1]

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Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml

Summary

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
nuclei
Positive Control
All human cells. Tissues.
Immunogen
Nuclei of human myeloid leukemia biopsy cells
Alternate Names
, HNA; HuNu

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
235-1
Mol. Weight of Antigen
70kDa & 80kDa

Key References

  • Rosario Sanchez-Pernaute et. al. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease. Brain. 2008; 131(8): 2127-2139.
  • Glaser R, Lu MM, Narula N, Epstein JA (2002) Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts. Circulation Jul 2;106(1):17-9. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease

Storage & Stability

Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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