Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

Printable Datasheet

HLA-DP/-DQ/-DR (MHC II) Antibody [CR3/43]

In Stock
Catalog Number Formulation Size Price
3115-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3115-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3115-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Tonsil stained with HLA- Pan Mouse Monoclonal Antibody (CR3/43).

Formalin-fixed, paraffin-embedded human Tonsil stained with HLA- Pan Mouse Monoclonal Antibody (CR3/43).

Formalin-fixed, paraffin-embedded human Tonsil stained with HLA- Pan Mouse Monoclonal Antibody (CR3/43).

Formalin-fixed, paraffin-embedded human Tonsil stained with HLA- Pan Mouse Monoclonal Antibody (CR3/43).

Flow Cytometric Analysis of Human Raji cells using HLA-Pan Mouse Monoclonal Antibody (CR3/43) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Flow Cytometric Analysis of Human Raji cells using HLA-Pan Mouse Monoclonal Antibody (CR3/43) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Immunofluorescent staining of Raji cells. HLA- Pan Mouse Monoclonal Antibody (CR3/43) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

Immunofluorescent staining of Raji cells. HLA- Pan Mouse Monoclonal Antibody (CR3/43) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

SDS-PAGE Analysis Purified HLA-Pan Mouse Monoclonal Antibody (CR3/43). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis Purified HLA-Pan Mouse Monoclonal Antibody (CR3/43). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of Ramos cell lysate using HLA-Pan Mouse Monoclonal Antibody (CR3/43).

Western Blot Analysis of Ramos cell lysate using HLA-Pan Mouse Monoclonal Antibody (CR3/43).

Western Blot Analysis of Human Spleen cell lysate using HLA-Pan Mouse Monoclonal Antibody (CR3/43).

Western Blot Analysis of Human Spleen cell lysate using HLA-Pan Mouse Monoclonal Antibody (CR3/43).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36kDa) and a chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immunology
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Lysosome membrane, trans-Golgi network membrane
Gene Name
HLA-DPB1
Positive Control
Raji cells. Tonsil, Ramos, Spleen or lymph node.
Immunogen
Cells from human tonsil
Alternate Names
HLA class II histocompatibility antigen, DP beta 1 chain, HLA class II histocompatibility antigen, DP(W4) beta chain, MHC class II antigen DPB1, HLA-DPB1; HLA-DQA1; HLA-DQB1; HLA-DRA; HLA-DRB1; HLA-DRB3; HLA-DRB4; HLA-DRB5;

Database Links

Entrez Gene ID
3115; 3117; 3122
SwissProt
P04440; P01908; P01909; P01920; P01903

Additional Information

Clone
CR3/43
Chromosome Location
6p21.3 (HLA-DP & HLA-DR)
Mol. Weight of Antigen
36kDa (' chain) and 27kDa (' chain)

Related Antibodies

View All HLA-DPB1 Antibodies

Functions

  • Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Key References

  • Gatter et al. 1982. J Clin Pathol. 35(11):1253-67.
  • Gatter et al. 1982. Semin Oncol. 9(4):517-25.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “HLA-DP/-DQ/-DR (MHC II) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales