Formalin-fixed, paraffin-embedded human Tonsil stained with Histone H1 Mouse Monoclonal Antibody (SPM256).

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Histone H1 Monoclonal Antibody (SPM256).

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Histone H1 Monoclonal Antibody (SPM256)

Confocal Immunofluorescence image of HeLa cells using Histone H1 Mouse Monoclonal Antibody (SPM256) (green-CF488). Phalloidin is used to label the membranes.

Flow Cytometric Analysis of HeLa cells. Histone H1 Monoclonal Antibody (SPM256) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

Western Blot Analysis of human heart tissue lysate using Histone H1 Mouse Monoclonal Antibody (SPM256).

SDS-PAGE Analysis Purified Histone H1 Monoclonal Antibody (SPM256). Confirmation of Purity and Integrity of Antibody.

Histone H1 (Pan Nuclear Marker) [SPM256]

Name: Histone H1 (Pan Nuclear Marker)
SKU: 3005-MSM1X
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Printable

Product Properties & Targets

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Research Areas

Apoptosis, Immuno Oncology

Isotype

IgG2a

Cellular Localization

N/A

Gene Name

H1-0, N/A

Mol. Weight of Antigen

30kDa

Immunogen

Nuclei of human leukemia biopsy cells

Database Links

Entrez Gene ID

3005

Unigene

226117; 97358

SwissProt

N/A

Additional Information

Clone

SPM256

Human Gene Symbol

Chromosome Location

3q21-q22; 6q21-q22; 22q13.1

Known Applications & Suggested Dilutions

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.