These antibodies are validated on HuProtâ„¢ Human Protein Microarrays containing over 21,000 full-length human proteins, including the specific target of interest. LEARN MORE
Trial Size Available
Carrier-Free Available
SDS-PAGE Analysis of Purified FOXI1 Mouse Monoclonal Antibody (PCRP-FOXI1-1F8). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence analysis of PFA-fixed HeLa cells. FOXI1 Mouse Monoclonal Antibody (PCRP-FOXI1-1F8) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow cytometric analysis of PFA-fixed HeLa cells. FOXI1 Mouse Monoclonal Antibody (PCRP-FOXI1-1F8) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
Formalin-fixed, paraffin-embedded human prostate stained. FOXI1 Mouse Monoclonal Antibody (PCRP-FOXI1-1F8) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
Analysis of HuProt™ Protein Array (21,000+ full-length human proteins) with FOXI1 Mouse Monoclonal Antibody (PCRP-FOXI1-1F8). Z-score: Signal strength (in SD above the mean) of antibody binding to each protein. S-score: Difference in Z-scores between the top target and the next best hit, indicating relative specificity. An antibody is considered specific if S ≥ 2.5. Example: Binding to protein X (Z = 43) vs protein Y (Z = 14) → S = 29.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
3
0
4
1
1
4
5
FOXI1 (forkhead box I1), also known as HFH3 (HNF-3/forkhead homolog 3), FKHL10 or FREAC6 (forkhead related transcription factor 6), is a member of the FOX family of transcription factors. The FOX family is a large group of proteins (consisting of at least 43 members) that share a common DNA binding domain termed winged-helix or forkhead domain. FOX transcription factors play important roles in development, differentiation, aging and hormone responsiveness. Localizing to the nucleus, FOXI1 functions as a transcription factor. Mice with mutated forms of FOXI1 show defects in ear development, implying that FOXI1 plays a significant role in the developmental pathway of ears and, in particular, the cochlea and vestibulum. FOXI1 is an upstream transcription regulator of Pendrin (a protein associated with deafness), suggesting a role for FOXI1 in the pathogenesis of Pendred syndrome (PS), a condition of nonsyndromic hearing loss and enlarged vestibular aqueduct (EVA).
Should a NeoBiotechnologies antibody not meet the experimental expectations outlined on our website or datasheet, please contact our Technical Support. We unconditionally guarantee our product.
Our team of scientists are ready to help you with expert recommendations or technical assistance related to applications or product use.
Information
© 2026 NeoBiotechnologies.  All Rights Reserved.
Monday-Friday: 8:00am – 8:00pm Pacific Time
