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Recombinant Ferritin, Heavy Chain (FTH) (Microglia Marker) Antibody [FTH/8700R]

In Stock
Catalog Number Formulation Size Price
2495-RBM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2495-RBM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2495-RBM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Mammalian ferritins consist of 24 subunits made up of 2 types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidationof FeI(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed; and oxidative stress, an important factor in the development of aging-related cataracts.

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm.
Gene Name
Positive Control
Human liver tissue. Liver tissue lysate.
Immunogen
Recombinant human FTH1 protein fragment (around aa 58-180) (exact sequence is proprietary)
Alternate Names
Ferritin heavy chain | Ferritin heavy chain, N-terminally processed, Cell proliferation-inducing gene 15 protein, Apoferritin; Cell proliferation-inducing gene 15 protein; Ferritin H subunit; FHC; FRIH; FTH1; FTHL6; Iron overload autosomal dominant; PIG15; Placenta immunoregulatory factor; Proliferation inducing gene 15 protein (PLIF)

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
FTH/8700R
Chromosome Location
11q12.3
Mol. Weight of Antigen
21kDa

Functions

  • Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed:9003196). Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (PubMed:9003196). Also plays a role in delivery of iron to cells (By similarity). Mediates iron uptake in capsule cells of the developing kidney (By similarity).

Key References

  • Y.Kaneko,T.Kitamoto,J.Tateishi,K.Yamaguchi. Ferritin immunohistochemistry as a marker for microglia. Acta Neuropathologica. 1989,Volume 79; 129-136.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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