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Fas (TNFRSF6) associated factor 1 Antibody [CPTC-FAF1-2]

In Stock
Catalog Number Formulation Size Price
11124-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
11124-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
11124-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis. Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved 'death domain' and belonging to the TNF receptor superfamily. TRADD, FADD and RIP are FAS/TNF-RI interacting proteins that contain a death domain homologous region (DDH). TRADD (TNF-RI-associated death domain) and FADD (FAS-associated death domain) associate with the death domains of both FAS and TNF-RI via their DDH regions, while RIP associates exclusively with FAS. An additional FAS interacting protein designated FAF1, for FAS-associated protein factor-1, binds with the cytoplasmic tail of wildtype but not LPR mutant FAS. When overexpressed in cells, FAF1 enhances the efficiency of FAS-mediated apoptosis. In contrast to TRADD, FADD and RIP, FAF1 lacks a DDH and cannot induce apoptosis independently of FAS activation.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Nucleus
Gene Name
Positive Control
breast carcinoma or colon carcinoma., HEK293, HeLa whole cell lysate. Human testis, MCF-7, prostate
Immunogen
Recombinant human full-length FAF1 protein
Alternate Names
FAS-associated factor 1, UBX domain-containing protein 12, UBX domain-containing protein 3A, CGI-03; FAS-associated factor 1; HFAF1s; UBX domain-containing protein 12; UBX domain-containing protein 3A

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
CPTC-FAF1-2
Chromosome Location
1p32.3
Mol. Weight of Antigen
75-80kDa

Functions

  • Ubiquitin-binding protein (PubMed:19722279). Required for the progression of DNA replication forks by targeting DNA replication licensing factor CDT1 for degradation (PubMed:26842564). Potentiates but cannot initiate FAS-induced apoptosis (By similarity).

Key References

  • Nagata, S. and Golstein, P. 1995. The FAS death factor. Science 267: 1449-1456

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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