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Cytochrome p450 Antibody [M12P4H2]

In Stock
Catalog Number Formulation Size Price
1571-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1571-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1571-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases, which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines, which are pre-mutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Endoplasmic reticulum membrane, Microsome membrane, Mitochondrion inner membrane
Gene Name
Positive Control
HepG2 cells. Liver.
Immunogen
Ovalbumin-conjugated synthetic peptide HIGFGCIPPR (C-terminal sequence)
Alternate Names
Cytochrome P450 2E1, 4-nitrophenol 2-hydroxylase, CYPIIE1, Cytochrome P450-J, 4-nitrophenol 2-hydroxylase; CPE1; CYP2Ecytochrome P450 2E1; CYPIIE1; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 ; Cytochrome P450-J; EC 1.14.13; EC 1.14.13.n7; EC 1.14.14.1; flavoprotein-linked monooxygenase; microsomal monooxygenase; P450C2E; P450-J; xenobiotic monooxygenase

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
M12P4H2
Chromosome Location
10q26.3
Mol. Weight of Antigen
~50-55kDa

Functions

  • A cytochrome P450 monooxygenase involved in the metabolism of fatty acids (PubMed:10553002, PubMed:18577768). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH
  • hemoprotein reductase) (PubMed:10553002, PubMed:18577768). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids (PubMed:10553002, PubMed:18577768). May be involved in the oxidative metabolism of xenobiotics (Probable).

Key References

  • Itoga, S., Nomura, F., Harada, S., Tsutsumi, M., Takase, S. and Nakai, T. 1999. Mutations in the exons and exon-intron junction regions of human cytochrome P-4502E1 gene and alcoholism. Alcohol. Clin. Exp. Res. 23: 13S-16S.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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