SDS-PAGE Analysis Purified CKBB Mouse Monoclonal Antibody (CKBB/6871). Confirmation of Purity and Integrity of Antibody

SDS-PAGE Analysis Purified CKBB Mouse Monoclonal Antibody (CKBB/6871). Confirmation of Purity and Integrity of Antibody

Printable Datasheet

Creatine Phosphokinase-BB (CK-BB) [CKBB/6871]

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Summary

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

Product Properties & Targets

Host
Mouse
Species Reactivity
Human
Isotype
IgG2b
Cellular Localization
Cytoplasm, Cytosol, Mitochondrion
Light Chain
kappa
Gene Name
CKB
Positive Control
HEK-293 and Cerebellum, SH-SY5Y
Immunogen
Recombinant human full-length CKBprotein
Alternate Names
Creatine kinase B-type, Brain creatine kinase, Creatine kinase B chain, Creatine phosphokinase B-type, BCK; Brain creatine kinase; Ckb; Creatine kinase B chain, Creatine kinase B-type; Creatine Kinase BB Isoenzyme; Creatine phosphokinase BB; Epididymis luminal protein 211; Epididymis secretory protein Li 29; HEL 211

Database Links

Entrez Gene ID
1152
Unigene
173724
SwissProt
P12277

Additional Information

Clone
CKBB/6871
Human Gene Symbol
CKB
Chromosome Location
14q32.32
Mol. Weight of Antigen
42kDa

Functions

  • Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed:8186255). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work (By similarity).

Known Applications & Suggested Dilutions

  • Optimal dilution for a specific application should be determined.

Key References

  • Lowe, M.T., et al. 2013. J. Cereb. Blood Flow Metab. 33: 1295-1306.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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