SDS-PAGE Analysis Purified CFTR Recombinant Rabbit Monoclonal Antibody (CFTR/7003R). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human spleen stained with CFTR Recombinant Rabbit Monoclonal Antibody (CFTR/7003R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with CFTR Recombinant Rabbit Monoclonal Antibody (CFTR/7003R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human kidney stained with CFTR Recombinant Rabbit Monoclonal Antibody (CFTR/7003R). Inset: PBS instead of primary antibody; secondary only negative control.

Printable Datasheet

CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) [CFTR/7003R]

Name: CFTR (Cystic Fibrosis Transmembrane Conductance Regulator)
SKU: 1080-RBM13
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

Recognizes a protein of 165-170kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.

Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Apical cell membrane, Cell membrane, Early endosome membrane, Endoplasmic reticulum membrane, Nucleus, Recycling endosome membrane

Gene Name

CFTR

Mol. Weight of Antigen

165-170kDa

Immunogen

Recombinant fragment (around aa550-850) of human CFTR protein (exact sequence is proprietary)

Database Links

Entrez Gene ID

1080

Unigene

489786; 621460; 661104

SwissProt

P13569

Additional Information

Clone

CFTR/7003R

Human Gene Symbol

CFTR

Chromosome Location

7q31.2

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.