Formalin-fixed, paraffin-embedded human tonsil stained with CD68 Rabbit Recombinant Monoclonal Antibody (C68/2908R). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

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Western Blot Analysis of human spleen tissue lysate using CD68 Rabbit Recombinant Monoclonal Antibody (C68/2908R).

Printable Datasheet

CD68 (Macrophage Marker) [C68/2908R]

Name: CD68 (Macrophage Marker)
SKU: 968-RBM10
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Cell membrane, Endosome membrane, Lysosome membrane

Gene Name

CD68

Mol. Weight of Antigen

110kDa

Immunogen

Recombinant full-length human LAMP4 / CD68 protein

Database Links

Entrez Gene ID

968

Unigene

647419

SwissProt

P34810

Additional Information

Clone

C68/2908R

Human Gene Symbol

CD68

Chromosome Location

17p13.1

Known Applications & Suggested Dilutions

Western Blot (1-2ug/ml)
,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Holness, C.L. et al. 1993. Blood 81: 1607-1613.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.