Western blot of CD3e in human Jurkat cells (1) absence and (2) presence of immunizing peptide. (3) Mouse thymus probed with CD3e Rabbit Polyclonal Antibody.

Western Blot of Jurkat cell lysate using CD3e Rabbit Polyclonal Antibody.

SDS-PAGE Analysis Purified CD3e Rabbit Polyclonal Antibody Confirmation of Purity and Integrity of Antibody.

Printable Datasheet

CD3e (T-Cell Marker) []

Name: CD3e (T-Cell Marker)
SKU: 916-RBP1
Availability: InStock

Polyclonals Primary Antibodies Rabbit Polyclonals

Summary

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Product Properties & Targets

Host

Rabbit

Applications

Western Blot

Species Reactivity

Human

Research Areas

Cardiovascular, Immuno Oncology

Isotype

IgG

Cellular Localization

Cell membrane

Light Chain

Kappa

Gene Name

CD3E

Positive Control

Jurkat cells. Tonsil and lymph node.

Immunogen

A synthetic peptide aa156-168 (ERPPPVPNPDYEP) from human CD3e

Alternate Names

T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD 3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex

Database Links

Entrez Gene ID

916

Unigene

3003

SwissProt

P07766

Additional Information

Human Gene Symbol

CD3E

Chromosome Location

11q23.3

Mol. Weight of Antigen

20kDa

Functions

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).

Known Applications & Suggested Dilutions

Western Blot (1-2ug/ml for 60 minutes at RT)
Optimal dilution for a specific application should be determined.

Key References

Cibull ML et. al. Histopathology, 1989, 15(6):599-605.
Mason DY et. al. Journal of Clinical Pathology, 1989, 42(11):1194-200.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “CD3e (T-Cell Marker)”