Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Rabbit Polyclonal Antibody.

Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Rabbit Polyclonal Antibody.

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CD15 / FUT4 (Reed-Sternberg Cell Marker) []

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Summary

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Product Properties & Targets

Host
Rabbit
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human, Mouse, Rat
Isotype
IgG
Cellular Localization
Golgi apparatus, Golgi stack membrane
Light Chain
Kappa
Gene Name
FUT4
Positive Control
Reed-Sternberg's cells in Hodgkin's lymphoma., U937 cells
Immunogen
Purified human neutrophils
Alternate Names
Alpha-(1,3)-fucosyltransferase 4, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, ELAM-1 ligand fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Galactoside 3-L-fucosyltransferase, 3 Fucosyl N Acetyl Lactosamine; Alpha (1,3) Fucosyltransferase; Alpha 13 fucosyltransferase FucT; ELAM Ligand Fucosyltransferase; ELFT; FCT3A; Fuc-TIV; Fucosyltransferase 4 Alpha 1 3 Fucosyltransferase Myeloid Specific; Fucosyltransferase 4; Galactoside 3 L Fucosyltransferase; Lewis X; LeX; SSEA1; Stage Specific Embryonic Antigen 1

Database Links

Entrez Gene ID
2526
Unigene
654379
SwissProt
P22083

Additional Information

Human Gene Symbol
FUT4
Chromosome Location
11q21
Mol. Weight of Antigen
220kDa

Functions

  • Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, Gal-beta(1->4)GlcNAc) glycan attached to N- or O-linked glycoproteins (PubMed:29593094, PubMed:1702034, PubMed:1716630). Robustly fucosylates nonsialylated distal LacNAc unit of the polylactosamine chain to form Lewis X antigen (CD15), a glycan determinant known to mediate important cellular functions in development and immunity. Fucosylates with lower efficiency sialylated LacNAc acceptors to form sialyl Lewis X and 6-sulfo sialyl Lewis X determinants that serve as recognition epitopes for C-type lectins (PubMed:29593094, PubMed:1716630). Together with FUT7 contributes to SELE, SELL and SELP selectin ligand biosynthesis and selectin-dependent lymphocyte homing, leukocyte migration and blood leukocyte homeostasis (By similarity). In a cell type specific manner, may also fucosylate the internal LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE (PubMed:1716630, PubMed:11278338).
  • Does not generate Lewis X antigens.

Known Applications & Suggested Dilutions

  • Flow Cytometry (2-4ug/million cells)
  • Immunofluorescence (2-4ug/ml)
  • Immunohistochemistry (Formalin-fixed) (5-10ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Key References

  • Hakomori, S. 1992. LeX and related structures as adhesion molecules. Histochem. J. 24: 771-776.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & Azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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