Formalin-fixed, paraffin-embedded human Uterus stained with Caldesmon Rabbit Polyclonal Antibody.

Formalin-fixed, paraffin-embedded Rat Uterus stained with Caldesmon Rabbit Polyclonal Antibody.

Western Blot Analysis of human ovary tissue lysate using Caldesmon Rabbit Polyclonal Antibody.

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Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) []

Name: Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker)
SKU: 800-RBP5
Availability: InStock

Polyclonals Primary Antibodies Rabbit Polyclonals

Summary

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This antibody recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Product Properties & Targets

Host

Rabbit

Applications

Flow Cytometry, Immunofluorescence, Western Blot

Species Reactivity

Human, Rat

Isotype

IgG

Cellular Localization

Cytoplasm, Cytoskeleton, Myofibril, Stress fiber

Light Chain

Kappa

Gene Name

CALD1

Positive Control

Blood vessels in all tissues, ovary, smooth muscle or leiomyosarcoma, Uterus

Immunogen

Recombinant human CALD1 protein fragment

Alternate Names

Caldesmon, CAD; CALD1; Caldesmon 1 Isoform 1; Caldesmon 1 Isoform 2; Caldesmon 1 Isoform 3; Caldesmon 1 Isoform 4; Caldesmon 1 Isoform 5; CDM; HCAD; LCAD; NAG22

Database Links

Entrez Gene ID

800

Unigene

490203

SwissProt

Q05682

Additional Information

Human Gene Symbol

CALD1

Chromosome Location

7q33

Mol. Weight of Antigen

120-150kDa

Functions

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).

Known Applications & Suggested Dilutions

Western Blot (1-2ug/ml)
Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
,Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Watanabe, K., Tajino, T., Sekiguchi, M. and Suzuki, T. 2000. H-Caldesmon as a specific marker for smooth muscle tumors. Comparison with other smooth muscle markers in bone tumors. Am. J. Clin. Pathol. 113: 663-668.
Frid MG, et al. Phenotypic changes of human smooth muscle cells during development: Late expression of heavy caldesmon and calponin. Dev Biol 1992; 153:185

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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