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Calcineurin A / PPP3CA Antibody [CALNA/2353]

In Stock
Catalog Number Formulation Size Price
5530-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5530-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5530-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Calcineurin A Mouse Monoclonal Antibody (CALNA/2353).

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Calcineurin A Mouse Monoclonal Antibody (CALNA/2353).

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Calcineurin A Mouse Monoclonal Antibody (CALNA/2353).

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Calcineurin A Mouse Monoclonal Antibody (CALNA/2353).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59kDa catalytic A subunit and a 19kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, MAPK Signaling, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Cell projection, Cytoplasm, Dendritic spine, Myofibril, Sarcolemma, Sarcomere, Z line
Gene Name
PPP3CA
Positive Control
HeLa, MCF-7 or A431 cells. Prostate, Thyroid or Pancreas.
Immunogen
Recombinant full-length human Calcineurin A protein
Alternate Names
Protein phosphatase 3 catalytic subunit alpha, CAM-PRP catalytic subunit, Calcineurin A alpha, Calmodulin-dependent calcineurin A subunit alpha isoform, Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, Calcineurin A alpha; Calcineurin A1; Calcineurin A; Calmodulin-dependent calcineurin A subunit alpha isoform; CALN; CALNA1; CAM-PRP catalytic subunit; CCN1; CNA alpha; CNA1; Ppp3ca; Protein phosphatase 2B catalytic subunit (PPP2B); Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform (PPP3CA)

Database Links

Entrez Gene ID
5530
SwissProt
Q08209

Additional Information

Clone
CALNA/2353
Chromosome Location
4q24
Mol. Weight of Antigen
62kDa

Related Antibodies

View All PPP3CA Antibodies

Functions

  • Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:15671020, PubMed:18838687, PubMed:19154138, PubMed:23468591, PubMed:30254215). Many of the substrates contain a PxIxIT motif and/or a LxVP motif (PubMed:17498738, PubMed:17502104, PubMed:22343722, PubMed:23468591, PubMed:27974827). In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation (PubMed:15671020). In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion (PubMed:18838687). Positively regulates the CACNA1B/CAV2.2-mediated Ca(2+) release probability at hippocampal neuronal soma and synaptic terminals (By similarity). Dephosphorylates heat shock protein HSPB1 (By similarity). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). In response to increased Ca(2+) levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation (PubMed:26248042). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138). May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins (PubMed:30611118). Dephosphorylates transcription factor TFEB at 'Ser-211' following Coxsackievirus B3 infection, promoting nuclear translocation (PubMed:33691586). Required for postnatal development of the nephrogenic zone and superficial glomeruli in the kidneys, cell cycle homeostasis in the nephrogenic zone, and ultimately normal kidney function (By similarity). Plays a role in intracellular AQP2 processing and localization to the apical membrane in the kidney, may thereby be required for efficient kidney filtration (By similarity). Required for secretion of salivary enzymes amylase, peroxidase, lysozyme and sialic acid via formation of secretory vesicles in the submandibular glands (By similarity). Required for calcineurin activity and homosynaptic depotentiation in the hippocampus (By similarity). Required for normal differentiation and survival of keratinocytes and therefore required for epidermis superstructure formation (By similarity). Positively regulates osteoblastic bone formation, via promotion of osteoblast differentiation (By similarity). Positively regulates osteoclast differentiation, potentially via NFATC1 signaling (By similarity). May play a role in skeletal muscle fiber type specification, potentially via NFATC1 signaling (By similarity). Negatively regulates MAP3K14/NIK signaling via inhibition of nuclear translocation of the transcription factors RELA and RELB (By similarity). Required for antigen-specific T-cell proliferation response (By similarity).

Key References

  • Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859.
  • Zeng H. et al. (2001) Cell 107:617.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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