Bromodeoxyuridine (BrdU) (Proliferation Marker) [BRD469]

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Summary

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Properties & Targets

Host
Mouse
Species Reactivity
All species
Isotype
IgG1, kappa
Cellular Localization
nuclear
Positive Control
Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.
Immunogen
Bromodeoxyuridine (BrdU) conjugated to KLH
Alternate Names
, Bromodeoxyuridine, BUdr

Database Links

Entrez Gene ID
Not Applicable
Unigene
Not Applicable
SwissProt
Not Applicable

Additional Information

Clone
BRD469
Mol. Weight of Antigen
307Da

Known Applications & Suggested Dilutions

  • Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood);Immunofluorescence (1:50-1:100);Optimal dilution for a specific application should be determined.

Key References

  • Welberg JW et. al. Journal of Clinical Pathology, 1990, 43(6):453-6.
  • Williams LS et. al. Cytometry, 1990, 11(4):490-7.

Storage & Stability

Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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