Bcl-X (Apoptosis Marker) [BCL2L1/4509R]

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Summary

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2δΌͺ or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241-amino acid protein which suppresses cell death. And Bcl-XS (short), a 178-amino acid protein lacking a 63-amino acid domain which functions as a dominant inhibitor of Bcl-2.

Product Properties & Targets

Host
Species Reactivity
Isotype
IgG
Gene Name
BCL2L1
Mol. Weight of Antigen
27kDa
Immunogen
Recombinant fragment (around aa1-100) of human BCL2L1 protein (exact sequence is proprietary)

Database Links

Entrez Gene ID
598
Unigene
SwissProt

Additional Information

Clone
BCL2L1/4509R
Human Gene Symbol
BCL2L1
Chromosome Location
20q11.21

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Hsu YT, et. al. Journal of Biological Chemistry, 1997, 272(21):13829-34.
  • Wolter KG, et. al. Journal of Cell Biology, 1997, 139(5):1281-92.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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