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Bcl-X (Apoptosis Marker) Antibody [SPM165]

Additional information

Catalog Number: 598-MSM2X
Reactivity:

, , ,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
598-MSM2X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
598-MSM2X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
598-MSM2X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Bcl-X (Apoptosis Marker) Antibody

Formalin-fixed, paraffin-embedded Hodgkin's lymphoma stained with Bcl-x Mouse Monoclonal Antibody (SPM165).

BCL2L1 Antibody in SDS-PAGE

BCL2L1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Bcl-2-like protein 1 Mouse Monoclonal Antibody (SPM165). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Mouse, Pig, Rat
Clone
SPM165
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Centrosome, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Cytosol, Microtubule organizing center, Mitochondrion inner membrane, Mitochondrion matrix, Mitochondrion outer membrane, Nucleus membrane, Secretory vesicle, Synaptic vesicle membrane
Gene Name
BCL2L1
Positive Control
HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma., Jurkat, K562
Immunogen
A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.
  • Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
  • Isoform Bcl-X(S) promotes apoptosis.
Key References
  • Hsu YT, et. al. Journal of Biological Chemistry, 1997, 272(21):13829-34.
  • Hsu YT, et. al. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94(8):3668-72.
  • Wolter KG, et. al. Journal of Cell Biology, 1997, 139(5):1281-92.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
598
SwissProt
Q07817
Chromosome Location
20q11.21
Mol. Weight of Antigen
27kDa
Alternate Names
Bcl-2-like protein 1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl-X, B cell lymphoma 2 like, BCL XL/S, Bcl-2-like protein 1, Bcl2-L-1, BCL2L, Bcl2l1, BCLX, BclXL, BclXs, Protein phosphatase 1 regulatory subunit 52 (PPP1R52)

Summary

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.

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