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Alpha-2-Macroglobulin Antibody [A2M/3622]

In Stock
Catalog Number Formulation Size Price
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes


伪-2-Macroglobulin (伪-2M) is a homotetrameric serum protein consisting of four identical subunits that form dimers through disulfide bonds. Initially, 伪-2M was characterized as a pan-proteinase inhibitor that was able to bait proteinases into cleaving specific peptide sequences on 伪-2M. This interaction induces a conformational change in 伪-2M, thus enabling it to trap the proteinase and further inhibit its activity. Subsequently, 伪-2M has been shown to function as a carrier protein and regulator of cytokines during inflammation. Circulating transforming growth factor 尾 (TGF尾) in serum is primarily bound to 伪-2M, which renders TGF尾 inactive. 伪-2M also binds to IL-6 and, thereby, increases the concentration of IL-6 near lymphocytes, hepatocytes and stem cells involved in mediating the inflammatory cascade. Mutations and deletions in the gene encoding 伪-2M are associated with an increased incidence of Alzheimer s disease (AD), which is consistent with the role of 伪-2M in mediating the clearance and degradation of A 尾, the major component of 尾-Amyloid deposits accumulated during AD.

Product Properties & Targets

Antibody Type
Species Reactivity
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Gene Name
Positive Control
Human liver or placenta tissues.
Human recombinant A2M protein fragment (around aa 604-748) (exact sequence is proprietary)
Alternate Names
Alpha-2-macroglobulin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; Alpha-2-M (A2M); CPAMD5

Database Links

Entrez Gene ID

Additional Information

Chromosome Location
Mol. Weight of Antigen


  • Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Key References

  • Wolf, B.B., et al. Am J Pathol. 1992 Jul; 141(1): 37 42.
  • d

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.


This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.


There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.


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