Free Shipping in the U.S. for orders over $1000.  Shop Now>>

AGO3 (Argonaute-3) / eIF2C3 Antibody [PCRP-AGO3-1C5]

In Stock
Catalog Number Formulation Size Price
192669-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
192669-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
192669-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml

Summary

This antigen is involved in binding and translation repression of short RNAs. This antigen is also known as argonaute RISC catalytic component 3.Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, P-body
Gene Name
Positive Control
HeLa, MCF7 or U-2OS cells.
Immunogen
Recombinant full-length human AGO3 protein
Alternate Names
Protein argonaute-3, Argonaute RISC catalytic component 3, Eukaryotic translation initiation factor 2C 3, argonaute 3; Argonaute3; eIF 2C 3; eIF-2C 3; eIF2C 3; Eif2c3; EIF2C3 protein; Eukaryotic translation initiation factor 2C 3; Eukaryotic translation initiation factor 2C3; FLJ12765; hAgo3; MGC86946; Protein argonaute-3

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-AGO3-1C5
Chromosome Location
1p34.3
Mol. Weight of Antigen
97.36kDa

Functions

  • Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity (PubMed:29040713).

Key References

  • Blackshaw S, et al. Nature methods 15.5 (2018 May): 330-338.

Storage & Stability

Antibody with azide store at 2 to 8 °C. Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “AGO3 (Argonaute-3) / eIF2C3 Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK