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Home >> Antibodies >> VISTA / B7-H5 / VSIR (Negative Regulator of Immune Response)

VISTA / B7-H5 / VSIR (Negative Regulator of Immune Response)

Mouse Monoclonal Antibody [Clone VISTA/3007]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Placenta stained with VISTA Monospecific Mouse Monoclonal Antibody (VISTA/3007).

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Formalin-fixed, paraffin-embedded human SqCC stained with VISTA Monospecific Mouse Monoclonal Antibody (VISTA/3007).

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SDS-PAGE Analysis Purified VISTA Monospecific Mouse Monoclonal Antibody (VISTA/3007). Confirmation of Integrity and Purity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using VISTA Monospecific Mouse Monoclonal Antibody (VISTA/3007).

Product Details

Synonyms

B7H5; DD1alpha; GI24; PD-1H; PDCD1 homolog; Platelet receptor Gi24; PP2135; Stress-induced secreted protein 1 (SISP1); V domain Ig suppressor of T cell activation (VISTA); V Set Immuno-Regulatory Receptor

Positive Control

Spleen or Placenta.

Known Applications & Suggested Dilutions

ELISA, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human VISTA protein

Cellular Localization

Cell Surface and Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

34kDa

Specificity & Comments

VISTA / GI24 is a transmembrane protein expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESC s, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V-type Ig-like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30kDa extracellular fragment plus a 25-30kDa membrane-bound fragment. VISTA is a negative checkpoint regulator and is expressed on myeloid cells, T-cells and human TILs (tumor infiltrating lymphocytes) on MDSCs (myeloid-derived suppressor cells) in the TME (tumor microenvironment). It is very likely both a ligand and receptor and is a promising target for cancer immunotherapy.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Lines, J.L., et al. Cancer Immunol Res. 2014;2(6):510-517.

Bioinformatics

  •  Entrez Gene ID: 64115
  •  Gene Symbol: VSIR
  •  SwissProt: Q9H7M9
  •  Chromosome Location: 10q22.1
  •  Unigene: 47382
NBT03007