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Home >> Antibodies >> Vinculin (Marker of Age-related Macular Degeneration)

Vinculin (Marker of Age-related Macular Degeneration)

Mouse Monoclonal Antibody [Clone VCL/2572]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Testis stained with VCL Mouse Monoclonal Antibody (VCL/2572).

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SDS-PAGE Analysis Purified VCL Mouse Monoclonal Antibody (VCL/2572). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of U-87 and THP-1 cell lysate using VCL Mouse Monoclonal Antibody (VCL/2572).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Vinculin Mouse Monoclonal Antibody (VCL/2572).

Product Details

Synonyms

CMD1W; CMH15; Epididymis luminal protein 114; HEL114; Metavinculin; MV; MVCL; VCL; VINC

Positive Control

U-87, THP-1 cells Human Bladder and Testis.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

117kDa

Specificity & Comments

Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Hwang, M.P., et al. 2016. Biomaterials 103: 22-32.

Bioinformatics

  •  Entrez Gene ID: 7414
  •  Gene Symbol: VCL
  •  SwissProt: P18206
  •  Chromosome Location: 10q22.2
  •  Unigene: 643896
NBT02572