Formalin-fixed, paraffin-embedded human Testis stained with VCL Mouse Monoclonal Antibody (VCL/2572).
SDS-PAGE Analysis Purified VCL Mouse Monoclonal Antibody (VCL/2572). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of U-87 and THP-1 cell lysate using VCL Mouse Monoclonal Antibody (VCL/2572).
Analysis of Protein Array containing more than 19,000 full-length human proteins using Vinculin Mouse Monoclonal Antibody (VCL/2572).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.