Recombinant Vinculin (Marker of Age-related Macular Degeneration) [VCL/8533R]



Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and Î �-Actinin. In addition, vinculin, Talin and Î �-Actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. Î �-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

Product Properties & Targets

Species Reactivity
Cellular Localization
Gene Name
Positive Control
A431|K562|NIH3T3|U-87 or THP-1 cells. Human bladder or testis.
Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary)
Alternate Names
Vinculin, Metavinculin, CMD1W; CMH15; Epididymis luminal protein 114; HEL114; Metavinculin; MV; MVCL; VCL; VINC

Database Links

Entrez Gene ID

Additional Information

Human Gene Symbol
Chromosome Location
Mol. Weight of Antigen


  • Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Known Applications & Suggested Dilutions

  • Western Blot (1-2ug/ml)
  • ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Hwang, M.P., et al. 2016. Biomaterials 103: 22-32.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.


This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.


There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.


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