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Home >> Antibodies >> TIGIT / VSTM3 / VSIG9 (Immune Checkpoint for Cancer)

TIGIT / VSTM3 / VSIG9 (Immune Checkpoint for Cancer)

Mouse Monoclonal Antibody [Clone TIGIT/3017]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with TIGIT-Monospecific Mouse Monoclonal Antibody (TIGIT/3017).

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SDS-PAGE Analysis Purified Monospecific Mouse Monoclonal Antibody to TIGIT (TIGIT/3017). Confirmation of Integrity and Purity of Antibody.

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Flow Cytometric Analysis of PFA-fixed MOLT4 cells. TIGIT-Monospecific Mouse Monoclonal Antibody (TIGIT/3017) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using TIGIT-Monospecific Mouse Monoclonal Antibody (TIGIT/3017).

Product Details

Synonyms

T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain containing 9 (VSIG9); V-set and transmembrane domain containing 3 (VSTM3); Washington University cell adhesion molecule (WUCAM)

Positive Control

MOLT-4, Prostate or Colon Carcinoma.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 22-141) of human TIGIT protein (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2c, kappa

Mol. Weight of Antigen

26kDa

Specificity & Comments

TIGIT is a checkpoint inhibitor which binds with high affinity to the poliovirus receptor (PVR), causing increased IL10 secretion, decreased IL12B secretion. TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4+ T cells. At the protein level, it is upregulated following the activation of these cells. Functionally, TIGIT is similar to CTLA4. The ligands for TIGIT include CD155 (signal abrogation) and CD226 (signal stimulation). It has been demonstrated to be upregulated on T cells in many cancers and is an immuno-oncology target for therapy.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Johnston et al., The Immunoreceptor TIGIT Regulates Antitumor and Antiviral CD8+ T Cell Effector Function, Cancer Cell (2014)
  2. Dougall WC, et al. TIGIT and CD96: New checkpoint receptor targets for cancer immunotherapy. Immunol Rev. 2017;276: 112-120

Bioinformatics

  •  Entrez Gene ID: 201633
  •  Gene Symbol: TIGIT
  •  SwissProt: Q4951
  •  Chromosome Location: 3q13.31
  •  Unigene: 421750
NBT03017