2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Thymidylate Synthase (5-FU Resistance Marker)

Thymidylate Synthase (5-FU Resistance Marker)

Recombinant Mouse Monoclonal Antibody [Clone rTYMS/1884]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

SDS-PAGE Analysis Purified TYMS Recombinant Mouse Monoclonal Antibody (rTYMS/1884). Confirmation of Purity and Integrity of Antibody.

img

Flow Cytometric Analysis of PFA-fixed MOLT4 cells. TYMS Recombinant Mouse Monoclonal Antibody (rTYMS/1884) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

img

Immunofluorescence Analysis of PFA fixed MOLT-4 cells labeling Thymidylate Synthase Recombinant Mouse Monoclonal (rTYMS/1884) followed by Goat anti-mouse IgG-CF488 (Green). Membrane is labeled with Phalloiden (Red).

Product Details

Synonyms

dTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase

Positive Control

MOLT-4 or Ramos cells. Testicular carcinomas.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IF         More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)

Cellular Localization

Nuclear & Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

36kDa

Specificity & Comments

It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Pestalozzi BC, et. al. Journal of Clinical Oncology, 1997, 15(5):1923-31
  2. Johnston PG, et. al. Journal of the National Cancer Institute, 1997, 89(4):308-13

Bioinformatics

  •  Entrez Gene ID: 7298
  •  Gene Symbol: TYMS
  •  SwissProt: P04818
  •  Chromosome Location: 18p11.32
  •  Unigene: 369762
NBT03812