2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Tenascin C (Stromal Marker for Epithelial Malignancy)

Tenascin C (Stromal Marker for Epithelial Malignancy)

Mouse Monoclonal Antibody [Clone T2H5]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Tenascin C Monoclonal Antibody (T2H5). Note staining of connective tissue.

Product Details

Synonyms

Cytotactin; Glioma-associated-extracellular matrix antigen; GMEM; GP 150-225; Hexabrachion (HXB); JI; Myotendinous antigen; Neuronectin; TNC

Positive Control

Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell and lung carcinomas. Staining of normal fibroblasts serves as internal positive control.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human breast carcinoma

Cellular Localization

Connective tissue matrix

Species Reactivity

Human. Does not react with Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

210kDa and 300kDa

Specificity & Comments

In Western blotting, it reacts with two bands of ~MW of 210kDa and 300kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Verstraeten AA, et. al. British Journal of Dermatology, 1992, 127(6):571-4.

Bioinformatics

  •  Entrez Gene ID: 3371
  •  Gene Symbol: TNC
  •  SwissProt: P24821
  •  Chromosome Location: 9q33
  •  Unigene: 143250
NBT00392