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Tenascin C (Stromal Marker for Epithelial Malignancy) Antibody [TNC/4806]

In Stock
Catalog Number Formulation Size Price
3371-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3371-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3371-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human lung stained with Tenascin C Mouse Recombinant Monoclonal Antibody (TNC/4806). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human lung stained with Tenascin C Mouse Recombinant Monoclonal Antibody (TNC/4806). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis of Purified Tenascin C Mouse Recombinant Monoclonal Antibody (TNC/4806). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified Tenascin C Mouse Recombinant Monoclonal Antibody (TNC/4806). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

In Western blotting, it reacts with two bands of ~MW of 210kDa and 300kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linked �hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Neuroscience, Angiogenesis
Isotype / Light Chain
IgG2c / Kappa
Cellular Localization
Connective tissue matrix
Gene Name
TNC
Positive Control
and lung carcinomas. Staining of normal fibroblasts serves as internal positive control (IHC).|Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast|squamous cell
Immunogen
Recombinant human full-length TNC protein
Alternate Names
Tenascin, Cytotactin, GMEM, GP 150-225, Glioma-associated-extracellular matrix antigen, Hexabrachion, JI, Myotendinous antigen, Neuronectin, Tenascin-C, Cytotactin; Glioma-associated-extracellular matrix antigen; GMEM; GP 150-225; Hexabrachion (HXB); JI; Myotendinous antigen; Neuronectin; TNC

Database Links

Entrez Gene ID
3371
SwissProt
P24821

Additional Information

Clone
TNC/4806
Chromosome Location
9q33
Mol. Weight of Antigen
210kDa and 300kDa

Related Antibodies

View All TNC Antibodies

Functions

  • Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (PubMed:19884327).

Key References

  • Verstraeten AA, et. al. British Journal of Dermatology, 1992, 127(6):571-4.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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