Home >> Antibodies >> Spectrin beta III (SPTBN2)

Spectrin beta III (SPTBN2)

Recombinant Rabbit Monoclonal Antibody [Clone SPTBN2/2894R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Western Blot Analysis of HeLa cell lysates using Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).

SDS-PAGE Analysis Purified Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human Pancreatic Cancer stained with Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).

Formalin-fixed, paraffin-embedded human Pancreatic Cancer stained with Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).

Western Blot Analysis of Human PANC-1 cell lysate using Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).

Flow Cytometric Analysis of HeLa cells using Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Western Blot Analysis of HeLa cell lysates using Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).

SDS-PAGE Analysis Purified Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human Pancreatic Cancer stained with Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).

Western Blot Analysis of Human PANC-1 cell lysate using Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).

Flow Cytometric Analysis of HeLa cells using Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

Beta III spectrin; SCA5; Spectrin beta chain brain 2; Spectrin beta non-erythrocytic 2; Spectrin non-erythroid beta chain 2; Spinocerebellar ataxia 5; SPTBN2

Positive Control

HeLa cells. Pancreas or Liver.

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
(ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human SPTBN2 fragment (aa356-475) (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

246kDa

Specificity & Comments

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.