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Home >> Antibodies >> Spectrin beta III (SPTBN2)

Spectrin beta III (SPTBN2)

Recombinant Rabbit Monoclonal Antibody [Clone SPTBN2/2894R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Western Blot Analysis of HeLa cell lysates using Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).

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SDS-PAGE Analysis Purified Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R). Confirmation of Integrity and Purity of Antibody.

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Formalin-fixed, paraffin-embedded human Pancreatic Cancer stained with Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).

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Formalin-fixed, paraffin-embedded human Pancreatic Cancer stained with Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).

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Western Blot Analysis of Human PANC-1 cell lysate using Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R).

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Flow Cytometric Analysis of HeLa cells using Spectrin beta III Rabbit Recombinant Monoclonal (SPTBN2/2894R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

Beta III spectrin; SCA5; Spectrin beta chain brain 2; Spectrin beta non-erythrocytic 2; Spectrin non-erythroid beta chain 2; Spinocerebellar ataxia 5; SPTBN2

Positive Control

HeLa cells. Pancreas or Liver.

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
(ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human SPTBN2 fragment (aa356-475) (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

246kDa

Specificity & Comments

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Speicher, D.W. 1986. J. Cell. Biochem. 30: 245-258.
  2. Coelman, T.R., et al. J.S. 1989. Cell Motil. Cytoskeleton 12: 225-247.

Bioinformatics

  •  Entrez Gene ID: 6712
  •  Gene Symbol: SPTBN2
  •  SwissProt: O15020
  •  Chromosome Location: 11q13.2
  •  Unigene: 26915
NBT02894